| 慢病毒载体介导GHRH基因在小鼠肌肉组织表达及对动物生长的影响 |
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| 引用本文： | 戴建威,章倩倩,刘松财,张明军,张永亮.慢病毒载体介导GHRH基因在小鼠肌肉组织表达及对动物生长的影响[J].中国生物工程杂志,2011,31(7):8-13. |
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| 作者姓名： | 戴建威 章倩倩 刘松财 张明军 张永亮 |
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| 作者单位： | 1. 广州医学院 广州 510182;
2. 华南农业大学动物科学学院 广州 510642;
3. 吉林大学畜牧兽医学院 长春 130062;
4. 中山大学生物工程中心 广州 510275 |
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| 基金项目： | 国家自然科学基金，广东省自然科学基金 |
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| 摘 要： | 人与动物体内生长激素受生长激素释放激素(Growth Hormone Releasing Hormone,GHRH)与生长激素抑制激素(Somatostatin,SST)两种因子共同调节,在体内表达外源GHRH,可以提高体内GH基础水平,进而达到促进体内GH释放,加速动物生长的效果.对慢病毒载体系统加以改造,使之成为C...
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| 关 键 词： | 生长激素 生长激素释放激素 双启动子 慢病毒 |
| 收稿时间： | 2011-01-05 |
| 修稿时间： | 2011-03-04 |
Lentiviral Vector Mediated GHRH Gene Expression in Mouse Muscle and the Effect on Growth of Animals |
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| DAI Jian-wei,ZHANG Qian-qian,LIU Song-cai,ZHANG Ming-jun,ZHANG Yong-liang.Lentiviral Vector Mediated GHRH Gene Expression in Mouse Muscle and the Effect on Growth of Animals[J].China Biotechnology,2011,31(7):8-13. |
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| Authors: | DAI Jian-wei ZHANG Qian-qian LIU Song-cai ZHANG Ming-jun ZHANG Yong-liang |
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| Institution: | 1 Department of Biochemistry,Guangzhou Medical College,Guangzhou 510182,China)(2 College of Animal Science,South China Agricultural University,Guangzhou 510642,China)(3 Department of Biochemistry,Veterinary College,Jinlin University,Changchun 130062,China)(4 Key Laboratory of Gene Engineering of the Ministry of Education,State Key Laboratory for Biocontrol, Sun Yat-Sen University,Guangzhou 510275,China) |
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| Abstract: | Overexpression of Growth hormone-releasing hormone (GHRH) can control the synthesis and secretion of the growth hormone (GH) from the hypophysis as well as regulate the GH concentrations in animals and humans. The lentiviral vector system were modified to be a CMV-SP two promoter vector. GHRH cDNAs were cloned into the vector and transfected 293T cells with other three plasmids to package LV-SP-GHRH pseudovirion. Muscle tissue of mice were injected with the virus particles and the animal weight growth were observed. At the same time viral safety was assessed by detection of whether the virus structural genes integrated into the genome of infected cells. The results showed that the body weight gain per day increased 153.85% compared to the control group at 28th day postinjection, and the genome of infected cells without virus structural gene integration. All the results indicated that lentiviral vector mediated GHRH gene can promote animal growth significantly and safely. |
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| Keywords: | Growth hormone Growth hormone releasing hormone Two promoter Lentivirus |
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