材料表面化学刺激对纤连蛋白构象及细胞的影响

生物材料的表面化学性质通过改变吸附蛋白的构象,影响细胞粘附铺展,产生不同的细胞行为.采用金-硫自组装单分子层技术(alkanethiol self-assembled monolayers,SAMs)构建了不同化学基团(-NH2、-COOH、-OH和-CH3)修饰的基底材料表面.运用X射线光能质谱(XPS)和接触角仪表...

Surface Chemistry Modulates Fibronectin Conformation and Control Cell Behavior

Biomaterial surface properties modulate protein adsorption and cell adhesion, proliferation, spreading to elicit diverse cellular responses in biomedical and biotechnological applications. Alkanethiol self-assembled monolayers (SAMs) presenting well-defined chemistries (NH₂, COOH, OH and CH₃) were employed to analyze the effects of surface chemistry on controlling fibronectin (FN) conformation as well as osteoblasts behaviors. X-ray photoelectron spectroscopy (XPS) is used to confirm the presence of well-formed monolayers of expected physicochemical characteristics, and the hydrophilicity of SAMs was characterized by water contact angle. Immunostaining were applied to indicate conformation of FN. In addition, the effects of SAMs with pre-coated FN on the cytoskeleton organization and proliferation were also examined. Water contact angle data reveals the-CH₃groups lead to a hydrophobic surface, while COOH, OH groups lead to a hydrophilic surface and NH₂groups a neutral surface. SAMs presenting terminal NH₂, COOH, OH and CH₃ functionalities modulated adsorbed FN conformation as determined through differences in the binding affinities of monoclonal antibodies raised against the central cell-binding domain,-OH surfaces exhibited a statistically greater HFN 7.1 positive area than the other surfaces. Then, the changes in osteoblasts proliferation and spreading correlated with the FN conformation.