检测纳米材料毒性的若干实验方法

纳米材料进入生命体和环境以后可能带来的生物安全问题需要定量的测定。现有的检测纳米材料生物安全性的方法大致可分为体内和体外实验两种，但是还没有证据表明既有的某个方法单独能作为一种检测所有纳米材料毒性的通用方法，也没有证据表明这些方法合在一起就能全面评估纳米材料的生物安全性。本文在总结既有的若干方法的同时，报道了一种基于rpsL基因的复制保真性来定量检测纳米材料毒性的方法。纳米材料对rpsL基因的体内体外复制过程保真性的影响均可方便地定量测定。

Several Approaches for Toxicity Assessment of Nanomaterials

Because of their specific physical chemical properties, nanomaterials need quantitative toxicity assessment when released into environment. Although there are some approaches to test the safety of nanomaterials, including in vitro and in vivo methods, there has been no evidence that a single standard way can work as a universal approach to evaluate toxicity of nanomaterials; even the combination of present approaches is still not enough for thourough assessment. While summarizing present methods, this study reported a new protocol to quantitatively detect perturbance on rpsL DNA replication fidelity induced by nanomaterials, both in vitro and in vivo.