| 硅藻变温发酵生产二十碳五烯酸的研究 |
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| 引用本文： | 曹小红,赵玉华,鲁梅芳,雷静,王春玲.硅藻变温发酵生产二十碳五烯酸的研究[J].中国生物工程杂志,2007,27(12):57-60. |
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| 作者姓名： | 曹小红 赵玉华 鲁梅芳 雷静 王春玲 |
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| 作者单位： | 天津科技大学食品工程与生物技术学院 天津科技大学食品工程与生物技术学院 天津科技大学 天津科技大学 天津科技大学食品科学与生物技术学院 |
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| 基金项目： | 天津市应用基础研究项目 |
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| 摘 要： | 本文考察不了同温度（10℃～30℃）对硅藻(Nitzschia laevis)合成二十碳五烯酸(EPA)的影响，对不同温度下的发酵过程进行动力学特性分析。在此基础上，提出了EPA合成的变温培养方法：延滞期及对数期初期温度控制在25℃，从对数期中期开始在20℃条件下进行培养。采用此变温培养进行发酵，EPA的含量和产量分别达到了6.00%和291.60 mg·L-1，较采用单一温度(25℃)发酵的最大值分别提高了24.07%和18.81%。
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| 关 键 词： | EPA 硅藻Nitzschia laevis 变温发酵 |
| 收稿时间： | 2007-08-09 |
| 修稿时间： | 2007年8月9日 |
Studies of Temperature Shift Fermentation for Eicosapentaenoic Acid Production by Nitzschia laevis |
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| CAO Xiao-hong,ZHAO Yu-hua,LU Mei-fang,LEI Jing,WANG Chun-ling.Studies of Temperature Shift Fermentation for Eicosapentaenoic Acid Production by Nitzschia laevis[J].China Biotechnology,2007,27(12):57-60. |
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| Authors: | CAO Xiao-hong ZHAO Yu-hua LU Mei-fang LEI Jing WANG Chun-ling |
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| Institution: | didi422@163.com |
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| Abstract: | Fermentation for Eicosapentaenoic Acid (EPA) production by Nitzschia laevis at various temperature between 10℃ and 30℃ was investigated and the dynamics characteristics during fermentation process were also analyzed. Based on the results, a varying temperature nursing method of two stage control strategy is proposed: During the firs stage, which comprises the delay phase and the initial index phase, the temperature is maintained at 25℃; then the temperature is shifted to 20℃ and kept up till the end of the fermentation process. By this method, a EPA content of 6.0% and a yield of 291.60 mg·L-1 have been gained. These are 24.07% and 18.81% higher than that of fixed temperature (25℃) fermentation, respectively. |
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| Keywords: | EPA Nitzschia laevis |
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