以BAC为基础的水痘-带状疱疹病毒的感染性克隆技术及其应用

水痘-带状疱疹病毒（VZV）属于疱疹病毒科α亚科，其原发感染为水痘，潜伏再度激活则引起带状疱疹。目前对其基因功能和疫苗的减毒机制尚不十分清楚。细菌人工染色(BAC)是一种新的用于大分子DNA克隆的载体系统，它具有容量大、遗传稳定、操作简单等优点。将VZV全基因组克隆至BAC系统构建成VZV的感染性克隆，并利用现代基因修饰技术可极大促进对该病毒的研究。就近年来以BAC为基础VZV感染性克隆技术的建立和应用做一综述。

Infectious Cloning Approach and Application for Varicella- Zoster Virus Based on Bacterial Artificial Chromosomes

Varicella-zoster virus (VZV) is a ubiquitous human alphaherpesvirus that causes varicella (chickenpox) during primary infection, can establish latency in sensory ganglia, and may reactivate to cause herpes zoster (shingles). Though VZV possesses the smallest genome among all human herpesviruses，little was known about its gene function and the attenuation mechanisms of VZV vaccine due to the difficulty of generating mutants to investigate gene function and the long period of time needed to establish pure clones using conventional techniques with mammalian cells. Recently，a completely new approach for full-length infectious clones of VZV based on bacterial artificial chromosomes(BACs) has been developed．This technique allows the maintenance，propagation and genetic modification of the viral genome as a BAC plasmid in E．coli，thus making the procedures fast，safe and effectivein prokaryotic cells．This technique also makes it possible for the reconstitution of viral progeny or mutants by transfection of the BAC plasmid into eukaryotic cells，thereby facilitating the an alysis of viral gene functions in the context of genome．The VZV BAC system also was useful as a vector for construction of recombinant live vaccines.