| 小菜蛾羧酸酯酶基因的克隆及其序列分析 |
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| 引用本文： | 王少丽,盛承发,乔传令.小菜蛾羧酸酯酶基因的克隆及其序列分析[J].中国生物工程杂志,2003,23(4):83-85. |
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| 作者姓名： | 王少丽 盛承发 乔传令 |
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| 作者单位： | 中国科学院动物研究所农业虫鼠害综合治理研究国家重点实验室 北京 100080 |
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| 基金项目： | 中国科学院知识创新项目资助 (KSCX2 0 1 0 2 ) |
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| 摘 要： | 利用CODEHOP设计简并引物,通过反转录多聚酶链式反应(RTPCR)克隆小菜蛾Plutellaxylostella抗性种群中抗性相关的羧酸酯酶基因,随机挑取测序5个阳性克隆进行测序,测序结果经过blastx比较,发现所获得的大约420bp的基因片断均为羧酸酯酶基因,与双翅目昆虫蚊子的氨基酸序列同源性达85%以上 。
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| 关 键 词： | 小菜蛾 简并引物 RT-PCR 羧酸酯酶基因 |
| 修稿时间： | 2003年1月15日 |
Cloning and Sequence Analysis of Carboxylesterase Gene in Diamondback Moth,Plutella xylostella (Lepidoptera:Plutellidae) |
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| Wang Shaoli Sheng Chengfa,Qiao Chuanling.Cloning and Sequence Analysis of Carboxylesterase Gene in Diamondback Moth,Plutella xylostella (Lepidoptera:Plutellidae)[J].China Biotechnology,2003,23(4):83-85. |
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| Authors: | Wang Shaoli Sheng Chengfa Qiao Chuanling |
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| Institution: | Institute of Zoology Chinese Academy of Sciences the State Key Laboratory of Integrated Management of Pest Insects and Rodents Beijing 100080 |
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| Abstract: | A pair of degenerate primers were designed by CODEHOP and were used to clone the carboxylesterase gene related with insecticide resistance in the resistant population of diamondback moth,Plutella xylostella (L.) using RT-PCR technique.Five positive clones were selected randomly and sequenced.After the blastx comparison,the partial cDNAs were confirmed that they were carboxylesterase gene and they have high identity (>85%) with Diptera insect mosquito.; |
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| Keywords: | Plutella xylostella Degenerate primer RT-PCR Carboxylesterase gene |
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