利用水稻启动子捕获系筛选水稻胚发育相关基因的初步研究

通过对2227个启动子捕获系中报道基因β-葡萄糖苷酸酶(β-glucuronidase,gus)表达活性检测，筛选到7个水稻胚中表达GUS活性的启动子捕获系，对其中编号为W9154的捕获系作了进一步分析。Southern杂交分析表明W9154是一个单拷贝T-DNA插入系，其T-DNA插入在水稻基因组第3号染色体上一个未知蛋白基因的第2个内含子中。对该未知蛋白基因上游调控序列生物信息学分析显示，其调控序列除含有TATA-box和CAAT-box等常见启动子元件外，还含有RY基序、E盒、G盒及AACA等种子特异性启动子的特征性元件。表达特征分析发现，编码上述未知蛋白的基因在野生型水稻的胚和茎中表达，这与捕获系W9154中GUS表达活性完全一致。这些表明启动子捕获系w9154捕获的候选基因可能是一个水稻胚发育相关基因。

Screening and Preliminary Analysis on an Embryo Development-related Gene from Promoter Trapping Line in Rice

Seven promoter trapping lines, which GUS activity were detected in embryo, were found by screening 2227 lines from an indica promoter trapping pool. Of which one line accession NO.W9154 was further analyzed.A single copy T-DNA insertion was indicated in the W9154 line by Southern blotting and its T-DNA inserted locus was located in the third intron of an unknown protein gene in chromosome 3 in rice. Analysis on the upstream regulation sequence of the trapped gene revealed that it contained seed-specific promoter elements, such as RY motif、E-box、AACA and so on, besides general promoter elements TATA-box and CAAT-box. Further analysis was conducted on expression pattern of the candidate gene, and it was found that the candidate was only expressed in embryo and stem, but not other tissues in wild type rice. This result was completely consistent with that of the GUS expression in the trapping line w9154.Taken together,the analyses indicated that the trapped gene of the line w9154 might be an embryo development-related gene in rice.