元素衡算和代谢衡算在类人胶原蛋白表达期的应用研究

应用元素衡算和代谢衡算方法，建立用重组大肠杆菌发酵生产类人胶原蛋白表达期的数学模型，并利用非线性优化法对模型中的未知参数进行估算。结果表明该模型与重组大肠杆菌的生长、代谢动力学模型一致，且模型的关键计算参数αh、αp和mx分别为1.173 molo C-mol-1、293.814 molo C-mol-1和17.878 molo C-mol-1oh-1。该模型能较好地预测重组类人胶原蛋白表达期中的宏观反应速率，且在类人胶原蛋白发酵表达期，必须通过控制葡萄糖的补料速率来控制菌体的生长，当?=0.04 h-1时，类人胶原蛋白的比生成速率达最大值。

Application of Element and Metabolism Balancing in the Production phase of Human-like collagen

On the base of element and metabolism balancing, the mathematic model of the human-like collagen expression phase with recombinant Escherichia coli BL21 was developed and the unknown parameters in the model were estimated with the method of nonlinear optimization. The model was in agreement with the growth kinetics and the metabolic kinetics, and the key calculated parameters of αh, αp and mx were 1.173 molo C-mol-1, 293.814 molo C-mol-1 and 17.878 molo C-mol-1oh-1 respectively. This model could preferably predict the macroscopic reaction rates, and in the synthesis phase of human-like collagen, the specific growth rate should be controlled at 0.04 h-1 with controlling glucose feeding rate to gain the highest specific production rate of human-like collagen.