噬菌体展示技术及其在肿瘤研究中的应用

噬菌体表面展示技术是一项特异性多肽或蛋白的筛选技术，它将随机序列的多肽或蛋白片段与噬菌体衣壳蛋白融合表达而呈现于病毒表面，被展示的多肽能保持相对独立的空间结构，使其能够与配体作用而达到模仿性筛选特异性分子表位，从而提供了高通量高效率的筛选系统。近年来噬菌体展示技术已广泛应用于肿瘤抗原抗体库的建立、单克隆抗体制备、多肽筛选、疫苗研制、肿瘤相关抗原筛选和抗原表位研究、药物设计、癌症检测和诊断、基因治疗及细胞信号转导研究等。就近年来噬菌体展示技术在肿瘤相关研究中的运用作以综述。

Phage Display Technology and Its Applications for Tumor Research

Phage display technology has been developed as a powerful tool for selecting polypeptides or proteins with desired biological and physicochemical properties from huge random peptide libraries. Fragments of foreign peptides or proteins that are expressed as fused proteins displayed on the phage surface can keep their relatively independent spatial structure and biological activity, so that they can interact with their ligands to mimic selection of specific molecular epitopes, thus providing an efficient high-throughput screening system. Phage display has been used to allow rapid identification of peptide ligands for a variety of target molecules by an in vitro selection process called “panning”. Phage display techniques can be widely exploited to construct tumorassociated antibody libraries, select polypeptides tumor-associated antigen, investigate antigen epitope and design vaccines and medicine, and are used especially as a tool for the diagnosis and treatment of tumor, gene therapy and tumor cell signal transduction research. Recent applications and advanced developments of phage display in cancer research are discussed in this article for the further reference to investigators.