丝状真菌蛋白表达系统研究进展*

丝状真菌以其优秀的表达分泌能力和良好的环境适应能力,使得其在蛋白质表达领域应用越来越广泛。近几十年来,通过诱变、培养优化及遗传改造等手段,使得包含曲霉属、木霉属、青霉属等在内的丝状真菌被开发成高效表达宿主。为促进丝状真菌蛋白表达系统的开发,结合作者的研究工作,对工业上丝状真菌表达宿主、蛋白质表达元件及其改造策略进行综述,并探讨了当前丝状真菌表达系统开发过程中的不足之处,为新型丝状真菌表达系统的研究提供参考和启示。

Recent Advances in Protein Expression System of Filamentous Fungi

Filamentous fungi, which with excellent protein secretion and environmental adaptability, are widely used in protein expression fields. In recent decades, the expression efficiency of filamentous fungi including Aspergillus sp., Trichoderma sp. and Penicillium sp. has been continuously increased by means of mutagenesis,culture optimization and genetic modification. In order to promote the development of filamentous fungal protein expression system,filamentous fungi expression hosts, protein expression elements and their modification strategies were reviewed . At the same time, the disadvantage in the development of filamentous fungal expression system were discussed to provide references and insights for the study of novel filamentous fungi expression systems.