| 白地霉Cryytococcus neoformans脂肪酶的双水相萃取 |
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| 引用本文： | 王俊华,杨洁,付建红,吴娜,石玉瑚,欧阳平凯.白地霉Cryytococcus neoformans脂肪酶的双水相萃取[J].中国生物工程杂志,2007,27(6):71-75. |
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| 作者姓名： | 王俊华 杨洁 付建红 吴娜 石玉瑚 欧阳平凯 |
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| 作者单位： | 新疆大学生命科学与技术学院 新疆大学生命科学与技术学院 新疆农科院微生物资源重点实验室 新疆大学生命科学与技术学院 |
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| 摘 要： | 本文研究了不同无机盐的双水相体系对白地霉脂肪酶的萃取分离效果，对PEG/（NH4）2SO4成相系统进行了系统的研究，通过考察体系PEG分子量、不同的无机盐、PEG浓度、（NH4）2SO4浓度、离子强度、pH值及（NH4）2SO4浓度对反萃取的影响，并通过正交实验进一步优化了实验条件，初步确定在PEG浓度15%，（NH4）2SO4浓度22.5%，pH8.0，不加NaCl的条件下进行双水相萃取，脂肪酶分离系数和纯化倍数分别为6.8和7.5，比活力达到40.3 U/mg蛋白。
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| 关 键 词： | 白地霉 脂肪酶 双水相萃取 |
| 收稿时间： | 2006-11-24 |
| 修稿时间： | 2006-11-242007-01-12 |
Lipase Extraction of Cryytococcus neoformans by Aqueous Two-phase System |
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| WANG Jun-hua,YANG Jie,FU Jian-hong,Wu Na,SHI Yu-hu,OUYANG Ping-kai.Lipase Extraction of Cryytococcus neoformans by Aqueous Two-phase System[J].China Biotechnology,2007,27(6):71-75. |
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| Authors: | WANG Jun-hua YANG Jie FU Jian-hong Wu Na SHI Yu-hu OUYANG Ping-kai |
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| Institution: | 1 .College of Life Science and Technology,Xinjiang University, Urumqi 830046,China; 2. Applied Microbiology institute,Xinjiang Academy of Agricultural Science, Urumqi 830012,China; 3. College of Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 211046,China |
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| Abstract: | A series of studies were conducted to establish a methodology for lipase extraction of Cryytococcus neoformans using aqueous two-phase system. After focusing on polyethylene glycol(PEG)/salt system and some other factors affected the extraction of aqueous two-phase system, The final methodology was obtained by the orthogonal experiment. In the case of maintaining PEG 15%(m/m),(NH4)2SO4 22.5%(m/m), pH8.0 without NaCl, the partition coefficient and purification multiple was 6.8 and 7.5 respectively and its specific activity was 40.3 U/mg protein. |
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| Keywords: | Cryytococcus neoformans Lipase Aqueous two-phase system extraction |
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