改构酸性成纤维细胞生长因子对小鼠胸腺细胞凋亡的影响

目的:探讨改构酸性成纤维细胞生长因子(MaFGF)对地塞米松(DEX)诱导小鼠胸腺细胞凋亡的保护作用。方法:利用地塞米松诱导小鼠胸腺细胞凋亡的模型,将实验分为空白对照组、DEX处理组、aFGF+DEX组和MaFGF+DEX组,采用DNA琼脂糖凝胶电泳和流式细胞仪两种分析方法,测定MaFGF对小鼠胸腺细胞凋亡的影响。结果:空白对照组凋亡率为168%,DEX处理后小鼠胸腺细胞出现明显的细胞凋亡,凋亡率为196%,aFGF和MaFGF处理后的细胞凋亡受到抑制,凋亡率分别下降到1595%和1293%,MaFGF效应强于aFGF,且以剂量依赖方式发挥作用。结论:MaFGF具有以剂量依赖方式的胸腺细胞保护作用。

Effect of Modified Acidic Fibroblast Growth Factor on Thymocyte Apoptosis Induced by Dexamethasone in Mouse

AimTo explore the effect of modified acidic fibroblast growth factor on thymocyte apoptosis induced by dexamethasone in murine.Methods The experimental groups include:(1)controls,(2)DEX only,(3)aFGF DEX,(4)MaFGF DEX.The apoptosis of thymocyte induced by dexamethasone was observed by DNA agarose gel electrophoresis.Flow cytometry was used to quantitate the percentage of apoptosis.Results Dexamethasone was shown to induce apoptosis in thymocyte cells.The percentage of apoptosic thymocyte cells induced by dexamethasone was obviously lower in those groups pretreated with 100ng/ml wild aFGF or MaFGF compared with groups treated with dexamethasone only,and the percentage of apoptosic thymocyte cells pretreated with MaFGF was much lower than pretreated with wild aFGF.It showed the increase of antagonistic effect of MaFGF on thymocyte apoptosis in 50,100,150,200ng/ml MaFGF pretreated groups.Conclusion Modified acidic fibroblast growth factor protects against dexamethasone-induced apoptosis in thymocyte in dose-dependent manner,which independ on its non-mitogenic activity. ;