| 运用均匀设计法优化rPA(K)在原核系统中的表达条件 |
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| 引用本文： | 罗惠霞,李敏,王玉炯,马春骥.运用均匀设计法优化rPA(K)在原核系统中的表达条件[J].中国生物工程杂志,2007,27(7):94-99. |
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| 作者姓名： | 罗惠霞 李敏 王玉炯 马春骥 |
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| 作者单位： | 西部特色生物资源保护与利用教育部院重点实验室 西部特色生物资源保护与利用教育部院重点实验室 西部特色生物资源保护与利用教育部院重点实验室 西部特色生物资源保护与利用教育部院重点实验室 |
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| 摘 要： | 运用均匀设计法对影响rPA(K)在大肠杆菌中表达的因素进行了优化设计，摸索出了对表达产物进行诱导表达的最佳优化表达条件。结果如下：溶解氧为90%，IPTG诱导时间为5.3 h、IPTG终浓度为0.1 mmol/L、培养基为HD、pH值为6.0、诱导温度为25?C。目的蛋白平均密度从0.16提高到0.48，是优化前的3倍。
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| 关 键 词： | rPA(K) 均匀设计 优化表达 |
| 收稿时间： | 2006-12-20 |
| 修稿时间： | 2006-12-202007-04-25 |
USING UNIFORM DESIGN TO OPTIMIZE EXPRESSIVE CONDITIONS OF rPA(K) IN E.COLI |
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| LUO Hui-xia,LI Ming,WANG Yu-jiong,MA Chung-ji.USING UNIFORM DESIGN TO OPTIMIZE EXPRESSIVE CONDITIONS OF rPA(K) IN E.COLI[J].China Biotechnology,2007,27(7):94-99. |
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| Authors: | LUO Hui-xia LI Ming WANG Yu-jiong MA Chung-ji |
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| Institution: | Key laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Yinchuan 750021 ,China |
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| Abstract: | We used Uniform Design to optimize some relative factors influencing the expressing of t-PA variant-rPA(K) in E.coli system, and found the best expressive conditions. They were(by using 300 mL culture fask): the volume of media was 25 mL, inducing time was 5.3 hours, pH was 6.0, the concentration of IPTG was 0.1 mmol/L, inducing time was 25?C, and best culture media was HD. After being optimized, the yield of expression had been improved from 0.16 to 0.48, and it was as 3 times as before. The results above will offer the basement for purification and renaturation of rPA(K). |
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| Keywords: | rPA(K) |
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