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高产谷胱甘肽酵母菌株的选育及其代谢通量分析
引用本文:俞志敏,徐凯,徐鹏,汤佳鑫,赵长新.高产谷胱甘肽酵母菌株的选育及其代谢通量分析[J].中国生物工程杂志,2008,28(7):110-115.
作者姓名:俞志敏  徐凯  徐鹏  汤佳鑫  赵长新
作者单位:大连工业大学 辽宁省发酵工程重点实验室 大连工业大学(原大连轻院)生物与食品工程学院 大连工业大学生物与食品工程学院
摘    要:利用UV和HNO2及其复合诱变处理S.cerevisiae 的原生质,筛选得到ZnCl2和半胱氨酸抗性菌株S.cerevisiae YZM-14(ZnCl2r,Cysr),其谷胱甘肽(GSH)产量(84.72mg/L)、生物量(7.63g/L)及胞内GSH含量(11.10mg/g)分别是出发菌株的2.79倍、1.63倍和1.71倍,且性状稳定。根据细胞比生长速率和GSH得率变化曲线,将GSH生物合成过程分为三个阶段,第二阶段诱变菌株与出发菌株相比PP途径代谢通量增加8.1 mmol/(g·h),GSH前体合成途径通量增加,且诱变菌株的有机酸分泌通量减少,提高了细胞的碳源利用效率,增大了GSH的生成。

关 键 词:谷胱甘肽  酿酒酵母  诱变育种  代谢通量分析  
收稿时间:2008-01-14
修稿时间:2008-04-22

Screening and Metabolic Flux Analysis of Glutathione-high-yielding strain from Saccharomyces cerevisiae
Abstract:The zinc chloride and cystine resistant strain of S. cerevisiae YZM-14(ZnCL2r, Cysr) was screened with the mutant processing of the protoplast of S. cerevisiae by combinative mutagens of ultraviolet and nitrite. The glutathione (GSH) production (84.72 mg/L), dry cell weight (7.63 g/L) and the intracellular GSH content (11.10 mg/g) of the mutant strain were about 2.79, 1.63 and 1.71 times than that of the initial strain. The biosynthetic process of GSH was divided into three phases according to the time course of the specific cell growth rate and GSH yielding coefficient. In the second phase, the metabolic flux of the pentose phosphate pathway and the GSH precursors biosynthetic pathway of the mutant strain increased by 8.1%, compared with that of the initial strain. Furthermore, the metabolic flux of the organic acids secretion of the mutant strain decreased. Through these mechanisms, the utilization efficiency of the carbon sources was enhanced and high production of GSH was obtained.
Keywords:Glutathione  Saccharomyces cerevisiae  Mutagenic breeding  Metabolic flux analysis
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