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葡萄蛋白质组学研究进展
引用本文:金良,陈尚武,马会勤.葡萄蛋白质组学研究进展[J].中国生物工程杂志,2010,30(10):100-107.
作者姓名:金良  陈尚武  马会勤
作者单位:1. 中国农业大学生物与技术学院 北京 100193; 2. 中国农业大学食品科学与营养工程学院 北京 100083
摘    要:人类基因组计划的完成标志着生命科学已进入后基因组时代,蛋白质组学的研究被提升到了前所未有的高度,蛋白质组学旨在阐明基因组所表达的真正执行生命活动的全部蛋白质的表达规律和生物功能。伴随葡萄基因组测序工作的完成,有关葡萄蛋白质组学的研究迅速发展。对近年来蛋白质组学在葡萄上的研究进行了综述,内容主要包括:葡萄蛋白质样品的提取制备,葡萄果实发育和品质形成过程中蛋白质组的变化,葡萄果皮、细胞壁、质膜等特定组织材料的蛋白质组研究,及蛋白质组学在葡萄逆境胁迫、体细胞胚的发生等方面的研究,并对葡萄蛋白质组学的发展趋势进行了展望。

关 键 词:葡萄  蛋白质样品制备  果实蛋白质组  果皮蛋白质组  胁迫蛋白质组  
收稿时间:2010-05-28
修稿时间:2010-08-07

Advances in Grape Proteomics
JIN Liang,CHEN Shang-wu,MA Hui-qin.Advances in Grape Proteomics[J].China Biotechnology,2010,30(10):100-107.
Authors:JIN Liang  CHEN Shang-wu  MA Hui-qin
Institution:1. College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, China; 2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
Abstract:With the completion of the human genome project, it has come into post-genomic era and now for the proteome. Proteomics aims at elucidating the expression, structure and function of all the proteins which are after all responsible for life’s activities. With the full sequencing of the grapevine genome, the grape proteomics research was significantly facilitated and be widely adopted. Recent development of grape proteomics were summarized. The contents include the protein sample extraction protocols for grape samples, results in berry development and quality formation, progress in berry skin, cell wall and plasma membrane proteomics, achievements in grapevine stress response and somatic embryo formation. In the end, the possible breakthrough in the near future were predicted.
Keywords:Grape  Protein sample preparation  Berry proteomics  Berry skin proteomics  Proteomics of grapevines under stress
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