外源蛋白在大肠杆菌中的表达定位策略

外源基因在大肠杆菌中表达是对基因重组技术的成功应用。外源基因在不同的大肠杆菌表达系统中表达产物可能定位于大肠杆菌空间结构的不同位置:胞质,胞质膜,胞周质,胞外膜和胞外培养基,五种表达定位方式各有其特点和用途 。

Strategies and Approaches to Expression and Localization of Heterologous Proteins in Escherichia coil

The expression of heterologous proteins is the most fascinating application of the recombinant DNA technique. The vectors from various expression systems of Escherichia coli provide the means for targeting a heterologous protein to any of the four subcellular compartments of the bacterial cell (cytoplasm, cytoplasmic membrane, periplasm and outer membrane) and extracellular medium. The production and localization of heterologous proteins or parts thereof in different compartments of Escherichia coli offers multiple applications. Of particular importance is that secretion of proteins is applicable for the production of proteins thereby enhancing protein activity and stability. Factors influencing folding, stability and export of extra|cytoplasmic proteins are also better understood.