| 增强型绿色荧光蛋白的真核表达和纯化 |
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| 引用本文： | 张梅,付远辉,何金生,陆燕燕,郑娴娴.增强型绿色荧光蛋白的真核表达和纯化[J].中国生物工程杂志,2011,31(5):99-103. |
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| 作者姓名： | 张梅 付远辉 何金生 陆燕燕 郑娴娴 |
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| 作者单位： | 1. 安徽医科大学免疫学教研室 合肥 230032;
2. 北京交通大学生命科学与生物工程研究院 北京 100044 |
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| 摘 要： | 根据编码增强型绿色荧光蛋白(enhanced green fluorescent protein,EGFP)的开放读码框(open reading frame,ORF)设计引物,PCR方法扩增出5'端带His标签的EGF PORF,利用杆状病毒表达系统构建表达EGFP基因的重组杆状病毒DNA分子,转染sf9细胞.取细胞...
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| 关 键 词： | 基因克隆 增强型绿色荧光蛋白 昆虫杆状病毒表达系统 |
| 收稿时间： | 2010-11-19 |
| 修稿时间： | 2011-03-10 |
Expression and Purification of Enhanced Green Fluorescent Protein Based on Baculovirus Expression System |
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| ZHANG Mei,FU Yuan-hui,HE Jin-sheng,LU Yan-yan,ZHENG Xian-xian.Expression and Purification of Enhanced Green Fluorescent Protein Based on Baculovirus Expression System[J].China Biotechnology,2011,31(5):99-103. |
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| Authors: | ZHANG Mei FU Yuan-hui HE Jin-sheng LU Yan-yan ZHENG Xian-xian |
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| Institution: | ZHANG Mei1 FU Yuan-hui2 HE Jin-sheng1,2 LU Yan-yan1 ZHENG Xian-xian1(1 Department of Immunology,Anhui Medical University,Hefei 230032,China)(2 College of Life Sciences & Bioengineering,Beijing Jiaotong University,Beijing 100044,China) |
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| Abstract: | Enhanced green fluorescent protein(EGFP) gene sequence with 5' end His tag was amplified by PCR.The recombinant baculovirus encoding EGFP was constructed and transfected into sf9 cells by Cellfectine reagent.The resultant fluorescence was observed by fluorescent microscope,and the expressed EGFP protein was purified by Ni column affinity chromatography and exploited as antigen to analyze specific humour immune response.The open reading frame(ORF) of EGFP with 5' end His tag was cloned successfully.The expre... |
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| Keywords: | Gene cloning Enhanced green fluorescent protein(EGFP) Baculovirus expression system |
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