重组B群脑膜炎球菌fHBP融合蛋白的表达和免疫原性研究

采用PCR构建编码B群脑膜炎球菌fHBP蛋白V<,1>变异型全长序列和V<,2>变异型C结构域抗原表位融合蛋白的基因片段.并克隆入pET30a<'(+)>载体,转化大肠杆菌BL21(DE3),在其实现表达,表达量约占菌体蛋白总量的30%.表达产物经离子交换层析纯化后,获得了纯度达到90%以上的融合蛋白.将融合蛋白免疫小...

Expression and Immunological Analysis of Recombinant Nesseria Meningitis Group B Recombinant fHBP Fusion Protein

Factor H-binding protein(fHBP) is a novel meningococcal vaccine candidate that elicits serum antibodies that activate classical complement pathway bacteriolysis and also inhibit binding of the complement downregulatory protein,factor H,to the bacterial surface.One limitation of fHBP as a vaccine candidate is antigenic variability,since antibodies to fHBP in the variant 1(V1) antigenic group do not protect against strains expressing V2 or V3 proteins,and vice versa.It can be defined three domains A,B and C,o...