一新用于生化反应器的在线细胞显微观察仪

针对生化反应器应用条件，提出了用于生化反应器的在线细胞观察仪的基本技术要求。在比较了国内外现有的细胞在线显微工作原理后，研制了一种基于暗视场的新的显微细胞观察仪，介绍了其关键技术及结构。另外，原位在线显微细胞观察仪应用于酿酒酵母和哺乳动物细胞HEK293细胞的培养试验，在线细胞计数结果与离线细胞计数和细胞干重相比较，均有很好的相关性，表明此仪器基本满足使用要求。

A Novel In-situ Microscope Applied in Biochemical Reactor

The technical requirements of in-situ microscope mounted in biochemical reactor were proposed based on biochemical reactor application. A novel in-situ microscope was designed and manufactured with dark field principle after all principles of in-situ microscopes used currently had been reviewed in detail, the designing principle and structure of a new in-situ microscope was then described. In addition, it was applied to monitor growth of baker yeast and mammalian cell HEK293, respectively. It indecated the cell amount obtained by in-situ microscope was a good agreement with dry cell weight and that determined using haemocytometer，suggested that the designed in-situ microscope could satisfy the requirements of high performance used in bioreactor .