大孔吸附树脂分离纯化地黄中梓醇工艺的研究

利用大孔吸附树脂分离提取地黄中梓醇。以地黄粗提液中梓醇含量为指标，高效液相色谱(HPLC)为含量测定方法，考察九种不同极性大孔吸附树脂对梓醇的吸附和解吸附性能，筛选出最佳树脂D101进行分离实验。结果表明，D101大孔吸附树脂的静态吸附容量为69.2mg/g干树脂，其吸附等温线符合Langmuir和Freundlich吸附等温式。采用5%乙醇作为洗脱剂，洗脱液减压浓缩后进行硅胶柱层析分离，氯仿：甲醇（8：2）梯度洗脱得到梓醇单体，纯度达90%以上，梓醇得率为6%。

Study on the separation and purification technology of catalpol from Rehmannia by macroporous adsorption resins

A separation technology of catalpol from Rehmannia with macroporpus adsorbent resins was investigated in this paper. The content of catalpol in the extract was determined by high performance liquid chromatography (HPLC). Nine different kinds of macroporous adsorbent resins were studied on the static capacity of adsorption and desorption, and D101 resin was best for the separation of the extraction solution of Rehmannia. The results showed that D101 resin had the highest static adsorption capacity of 69.2mg/g dry resin and its isotherm curve can be well described by Langmuir and Freudlich equation. The 5% ethanol elution on removal of the solvent under reduced pressure provided a brown powder, which was subjected to an open column chromatography on silica gel eluted with a CHCl3–MeOH gradient. The fraction eluted with CHCl3–MeOH (8：2) was identified as catalpol and the purity of the compound was more than 90% purity by HPLC analysis. The yield of this separation technology was 6%.