豆血红蛋白基因lba的克隆及其转化衣藻叶绿体

降低细胞内的氧气含量是提高莱茵衣藻产氢效率的重要手段之一。本研究首次尝试将豆血红蛋白基因lba转入衣藻叶绿体中表达，利用豆血红蛋白具有与氧可逆结合的特性，期望降低转基因衣藻细胞内的氧气含量，达到提高衣藻产氢效率的目的。实验结果证明，lba成功转入到衣藻叶绿体中，且对其生长没有产生显著影响，这为下一步调控Lba在衣藻叶绿体中表达活性和提高衣藻产氢效率奠定了实验基础。

Cloning and Transformation of lba Gene of Glycine max into Chloroplast of Chlamydomonas reinhardtii

To decrease the oxygen content in the cell is a key method to improve hydrogen production in Chlamydomonas reinhardtii. To achieve this goal, we developed a new approach by transforming the leghemoglobin gene lba, which has high affinity to oxygen, into the chloroplast of C. reinhardtii to get a low dissolved oxygen in the cell and result into improvement of H2ase activity and H2 yield. The results showed that lba was successfully transformed into the chloroplast of C. reinahrdtii strain 849 and did not affect its growth significantly. This work paved the road for further regulation of lba expression in the chloroplast to improve of hydrogen production of C. reinahrdtii.