| 脑膜炎奈瑟氏菌外膜蛋白NhhA的克隆表达及免疫学分析 |
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| 引用本文： | 陈柳,李健峰,徐维明,戴宗祥,李智华,孙强明.脑膜炎奈瑟氏菌外膜蛋白NhhA的克隆表达及免疫学分析[J].中国生物工程杂志,2010,30(4):8-12. |
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| 作者姓名： | 陈柳 李健峰 徐维明 戴宗祥 李智华 孙强明 |
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| 作者单位： | 北京协和医学院/中国医学科学院 医学生物学研究所 昆明 650118 |
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| 摘 要： | 通过克隆脑膜炎奈瑟氏菌NhhA基因GNA0992于原核表达载体pET 20b，在大肠杆菌BL21（DE3）中实现了可溶性表达，表达量约占菌体蛋白总量的20%。经初步纯化后免疫小鼠，对其免疫原性进行了初步分析。结果显示，经3次腹腔免疫，血清IgG滴度达到23186，同时杀菌力实验显示NhhA能诱导针对B群脑膜炎奈瑟氏菌的补体依赖的杀菌反应。证明了NhhA是一种良好的抗原，为疫苗开发的蛋白靶标筛选工作奠定了基础。
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| 关 键 词： | 脑膜炎奈瑟氏菌 NhhA 原核表达 免疫原性 |
| 收稿时间： | 2009-11-18 |
| 修稿时间： | 2009-12-08 |
Clone, Expression and Immunological Analysis of Neisseria meningitidis Outer Membrane Protein NhhA |
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| CHEN Liu,LI Jian-feng,XU Wei-ming,DAI Zong-xiang,LI Zhi-hua,SUN Qiang-ming.Clone, Expression and Immunological Analysis of Neisseria meningitidis Outer Membrane Protein NhhA[J].China Biotechnology,2010,30(4):8-12. |
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| Authors: | CHEN Liu LI Jian-feng XU Wei-ming DAI Zong-xiang LI Zhi-hua SUN Qiang-ming |
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| Institution: | Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China |
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| Abstract: | Neisseria meningitidis gene GNA0992 was cloned into the prokaryotic expression vector pET-20b,and soluble expressed in E.coli BL21 (DE3).Purified NhhA stimulated a significant antigen-specific humoral response following three intraperitoneal immunization,while bactericidal assay showed that NhhA can elicit antibodies capable of inducing bactericidal activity against group B N.meningitidis strains.The results indicate that NhhA is a good candidate for vaccine development. |
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| Keywords: | Neisseria meningitides NhhA Prokaryotic expression Immunological property |
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