用YO-PRO-1和PI联合染色定量检测细胞凋亡

经不同浓度staurosporine处理诱导凋亡的G7细胞样品,分别用YO-PRO-1/PI和AV/PI进行荧光染色,借助流式细胞仪检测凋亡情况,将两种检测方法得到的结果进行统计学分析显示,二者有显著的相关性(r=0.9659,P<0.01),且没有显著性差异(P<0.05);另外,上述凋亡细胞样品经YO-PRO-1/PI染色后在荧光显微镜下计数凋亡细胞比例的结果与AV/PI流式细胞仪的检测结果也有显著的相关性(r=0.9903,P<0.01),且没有显著性差异(P<0.05)。以上这些结果表明,用YO-PRO-1/PI对细胞进行染色、借助流式细胞仪和荧光显微镜均能准确地检测细胞凋亡,可替代AV/PI流式细胞仪方法用于细胞凋亡的检测。

Detection of Apoptosis by YO-PRO-1/PI Staining

Apoptosis in G7 cells was induced by different concentrations of staurosporine. Cells were stained with YO-PRO-1/PI and AV/PI respectively and apoptosis was detected by flow cytometry. The data obtained were analysed statistically. Excellent correlation values (r=0.9659, P<0.01) and no significant difference (P<0.01) were found between YO-PRO-1/PI staining and AV/PI staining. In addition, similar statistics were obtained between YO-PRO-1/PI-stained fluorescence microscopy and AV/PI-stained flow cytometry. The results indicated that apoptosis could be accurately detected by YO-PRO-1/PI stained flow cytometry and fluorescence microscopy and that YO-PRO-1/PI staining could substitute for AV/PI staining in the detection of apoptosis.