香蕉BTB/POZ域基因的克隆及表达分析

利用抑制缩减杂交文库从香蕉中分离获得一个cDNA片段,结合RACE技术获得该基因的全长序列,命名为MaBTB基因,该cDNA的ORF全长1 080个碱基。通过Blastx同源性分析结果显示该基因的氨基酸序列与水稻OsBTB、苜蓿MtBTB/POZ、拟南芥AtBPM4、葡萄VvBTB、玉米ZmBTB 、松树PsBTB基因的氨基酸序列同源性很高,分别为86%、85%、84%、84%、86%和87%。遗传进化树分析发现该基因与苜蓿MtBTB/POZ和拟南芥AtBPM4基因亲缘关系较近。用RT-PCR的方法研究该基因在不同胁迫处理下的表达特性,结果表明该基因在盐、乙烯和紫外线处理后其表达量逐渐增强;低温处理和香蕉尖孢镰刀菌4号生理小种处理后该基因表达量开始降低,随后逐渐升高;伤害处理后该基因的表达量没有明显变化。

Cloning and Expression Analysis of BTB/POZ Gene in Banana

A full length cDNA named MaBTB (BR-C, ttk and bab or pox virus and zinc finger), which contained 1 080bp with an open reading frame, was isolated by SSH combined with RACE technology. Sequence analysis revealed that this gene shared high identities of 86%、85%、84%、84%、86% and 87% to those of BTB/POZ from Oryza sativa(OsBTB), Medicago truncatula(MtBTB/POZ), Arabidopsis thaliana(AtBPM4), Vitis vinifera(VvBTB), Zea mays (ZmBTB)and Picea sitchensis(PsBTB)), respectively. Phylogenetic analysis showed that MaBTB was closely related to Medicago truncatula (MtBTB) and Arabidopsis thaliana (AtBPM4).The expression feature of MaBTB was investigated by RT-PCR. The result showed that under salt, ethylene and ultraviolet radiation treatment, the expression of MaBTB gradually increased. The accumulation of MaBTB decreased and then gradually increased with cold and FOC race 4 treatment. However, the accumulation of MaBTB was not distinct variety by wounding treatment.