微生物生态学中分子生物学方法及T-RFLP技术研究

根据微生物基因 (DNA)多态性来研究微生物的多样性 ,是建立在多聚酶链式反应 (PCR)基础之上分子生物学的新方法 ,克服了传统微生物培养方法的限制。从理论、实验及应用角度出发 ,介绍了几种在微生物生态学中应用较为广泛的分子生物学技术 ;详细阐述了微生物生态学中分子生物学的一种新研究方法———末端限制性片段长度多态性 (T -RFLP)技术 ,该技术作为一种研究微生物群落特征的理想方法已经越来越受到人们的重视。

Molecular Approaches Studying on Microbial Ecology and T-RFLP Technique

The molecular approaches that base on PCR have overcome the confinement of conventional culture dependent methods.These methods depending on the diversity of DNA study the diversity of microbial.A few of wildly used molecular approaches in the microbial ecology are presented briefly in this article.Terminal restriction fragment length polymorphism(T RFLP) is a resent molecular approach that can assess subtle genetic differences between strains as well as provide insight into the structure and function of microbial communities.T RFLP introduced in this article has been drawing more and more attention of researchers.;