牛分离精子对其IVF胚胎染色体影响的研究

本研究采用传统的细胞遗传学方法，研究了由流式细胞仪分离的、染色未分离的及作为对照用的未染色未分离的分别来自于3头公牛的精子IVF（in vitro fertilization, IVF)后产生的6~8 d囊胚的染色体异常情况，以确定流式细胞仪分离精子的过程及染色对胚胎染色体异常的影响。结果显示，分离精子、染色未分离精子和未染色未分离精子的胚胎中，染色体组成为异常，即嵌合体的胚胎分别占40.7%（59/145）、35.8%（38/106）和37.0%（37/100），三者染色体异常的比例无显著差异。胚胎染色体异常的频率在不同公牛之间存在差异（33.0% 比 44.6%）（p<0.05）。本研究的结果证明，染料和分离过程没有影响精子的DNA进而影响胚胎的染色体组成；胚胎染色体异常的频率在不同公牛之间存在差异。

Effect of flow cytometrically-sorted sperm on chromosomes of bovine blastocysts produced in vitro

The chromosome abnormalities of blastocysts (6 d ~ 8 d ) produced by IVF(in vitro fertilization) with flow cytometrically-sorted, stained and unsorted sperm from 3 bulls were investigate in this study, which determine if the sorting procedure and staining cause the chromosomal abnormality of embryos. The results revealed that there were no significant differences in chromosomal abnormality (mosaic) rates of blastocysts derived from sorted, stained unsorted and unsorted sperm (40.7%, 59/145; 35.8%, 40/106 and37.0%,37/100, respectively); Chromosomal abnormality rates were variant among bulls (30.0% vs 44.6%; p<0.05); These results indicated that: 1) neither sorting procedure nor staining affect the chromosomal composition of embryos; The chromosomal abnormality rates of embryos were variable among bulls.