植物异黄酮合酶研究进展

植物异黄酮是一类具有增强植物抗病、诱导根瘤形成以及预防激素相关肿瘤发生、缓解女性更年期综合症的活性次生代谢产物,其合成关键酶是异黄酮合酶(IFS)。介绍了IFS的催化机理、基因克隆与表达调控的研究进展,讨论了开展IFS代谢工程研究对提高植物抗病虫害能力和改善农作物营养保健功效的意义。

Progress in Isoflavone Synthase

Isoflavones are a group of secondary metabolites common to leguminous plants that play roles in plant defense and nodulation and have many benefits to human health including relief of menopausal symptoms, reduction of osteoporosis and lowering risk of certain hormone-related cancers. Isoflavone synthase (IFS), which is a member of cytochrome P450, is the key enzyme in isoflavone biosynthesis. The progress in catalytic mechanism of IFS enzyme, cloning and functional characterization of IFS, and expression and regulation of IFS genes was introduced. The significance of metabolic engineering of IFS gene expression and regulation to increase isoflavone biosynthesis was discussed.