拟南芥VHA-c3启动子的GUS基因融合表达

VHA-c3基因是拟南芥液泡H+-ATPase c亚基的5个同源基因之一，已有研究结果表明其与植物抗非生物胁迫有关。本论文在克隆不同长度VHA-c3基因上游调控序列的基础上，利用GUS报告基因，研究了VHA-c3基因的植物组织及器官定位。结果表明：VHA-c3起始密码子上游772bp的序列内存在着VHA-c3基因的基本启动子元件，可指导基因组成型表达在拟南芥的叶片、表皮毛、叶柄、根、雄蕊、柱头和萼片；在VHA-c3起始密码子ATG上游2812bp-2234bp片段和1496bp-772bp片段中各存在一个负调控元件，在2234bp-1496bp片段中存在一个正调控元件，它们的存在可控制基因在气孔中的表达。

Localization of Arabidopsis VHA-c3 Promoter with GUS-Fusion

VHA-c3 gene is one of the five homologous genes which belong to the subunits of vacuole H+-ATPase in Arabidopsis thaliana. Former reports indicated that it is related with resistance to abiotic stress in plants. In this article, different fragments of the upstream regulation sequences of Arabidopsis VHA-c3 gene were cloned, and fused with GUS reporter gene, to study the localization of VHA-c3 gene. The results showed that: The essential element for the basal activity of VHA-c3 is located within the -722bp fragments upstream of the translation start codon of VHA-c3, it can guide genes' constitutive expression in leaf blade, trichome, petiole, root, stamen, stigma and sepal in Arabidopsis; And there is two negative regulatory elements located in -2812bp to -2234bp and -1496bp to -772bp respectively, and an up-regulating cis-element from -2234bp to -1496bp fragment of VHA-c3 gene, which may control the gene's expression in guard cell.