| 太空环境改变生物工程细胞CHO(dhfr- |
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| 引用本文： | 徐梅,向青,房青,李红艳,徐波,高福云,唐劲天.太空环境改变生物工程细胞CHO(dhfr-[J].中国生物工程杂志,2004,24(11):22-25. |
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| 作者姓名： | 徐梅 向青 房青 李红艳 徐波 高福云 唐劲天 |
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| 作者单位： | 中日友好医院临床医学研究所肿瘤学分子生物学研究室, 北京 100029 |
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| 基金项目： | 国家科技部基础平台资助项目 ( 2 0 0 4年度 ) |
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| 摘 要： | 目的 :了解太空诱变对生物工程细胞CHO(dhfr----
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| 关 键 词： | 太空 CHO(dhfr- |
| 收稿时间： | 2004-06-01 |
| 修稿时间： | 2004年6月1日 |
Effects of Spaceflight on CHO(dhfr-)Cells Growth Characteristics |
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| XU Mei,XIANG Qing,FANG Qing,LI Hong-yan,XU Bo,GAO Fu-yun,TANG Jin-tian.Effects of Spaceflight on CHO(dhfr-)Cells Growth Characteristics[J].China Biotechnology,2004,24(11):22-25. |
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| Authors: | XU Mei XIANG Qing FANG Qing LI Hong-yan XU Bo GAO Fu-yun TANG Jin-tian |
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| Abstract: | Objective: To investigate the effects of spaceflight on CHO(dhfr-) cells growth characteristics Methods: CHO(dhfr-) cells were loaded and flight 18days on the No.18 recovered satellite. All survival cells were monocoloned and 159 cell lines were obtained. 3 cell lines selected randomly were used as cell models, the experimental methods such as cell morphological observation, MTT assay, FACS and 3H incorporation were used for analysis of cell growth characteristics. Results: Comparing with the control group, CHO(dhfr-) cells appeared multiple cell morphological changes and the cell number of G1 phase was increased markedly (p<0.05). Growth rate ,biosynthesis of nucleic acid and protein in mutated cells were varied without specifically disciplinarian. Conclusion: The data suggested that effects of spaceflight on growth characteristics were complicated and multiplex in CHO(dhfr-) cells.; |
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| Keywords: | Outer space CHO(dhfr-) cell Growth charateristic |
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