携带cip基因的酿酒酵母对自由生活线虫Panagrellus redivivus生长繁殖的影响

Photorhabdus luminescens细菌与昆虫病原异小杆属Heterorhabditis线虫专性共生。初生型共生细菌产生两种胞内晶体蛋白CipA and CipB,为共生线虫提供营养。为探索Cip蛋白是否对自由生活的全齿复活线虫Panagrellus redivivus具有类似的营养功能,建立了Cip蛋白的重组酿酒酵母表达体系,并用于饲喂无菌的P. redivivus线虫J1幼虫。重组酿酒酵母表达的Cip蛋白能为线虫所利用,表现为营养支持作用,体现为线虫生长发育速度的加快以及繁殖能力的提高,说明Cip蛋白能为此种自由生活线虫提供营养来源。

Development of a Free-living Nematode Panagrellus redivivus in Saccharomyces cerevisiae with cip Genes

CipA and CipB are two types of intracellular crystalline inclusion proteins produced by Photorhabdus luminescens bacteria, which are symbionts of entomopathogeic Heterorhabditis nematodes. To understand the biological function of these proteins for free living Panagrellus redivivus nematodes, recombinant Saccharomyces cerevisiae expression system of Cip proteins were constructed and the resulting yeast cells were used to feed the sterile J1 juveniles of P. redivivus. In recombinant yeast cells with CipA and/or CipB, the nematodes developed about 24 h faster than those in the yeast ceils without Cip proteins. This promotion was reflected in two aspects: to short the cycle time and to enhance the reproductive ability of P. redivivus nematode. It means that the nutrient sources from entomopathogeic nematodes are acceptable by this free-living nematode.