miR-106b转基因小鼠的建立

目的:建立miR-106b转基因小鼠模型,探讨其在阿尔茨海默病(Alzheimer’s disease, AD)发病中的作用。方法:构建miR-106b表达载体,显微注射法建立miR-106b转基因小鼠。PCR鉴定转基因小鼠的基因型,real time RT-PCR检测miR-106b转基因小鼠脑组织中miR-106b的表达情况,Western blot检测miR-106b转基因小鼠脑组织中TGFBR2蛋白的表达。结果:构建了高表达miR-106b转基因小鼠;与对照相比,miR-106b转基因小鼠脑组织中TGFBR2蛋白的表达升高。结论:miR-106b转基因小鼠的建立为研究该microRNA在AD发病中的作用提供了工具。

Establishment of miR-106 b Transgenic Mouse

Objective:To establish a miR-106b transgenic mouse and investigate its effect on the development of Alzheimer’s disease (AD). Methods:The expression vector of miR -106b was constructed. The transgenic mouse was produced by microinjection and genotype was detected by PCR. The expression levels of miR-106b were detected by real time RT-PCR. The protein levels of TGFBR2 were detected by Western blot. Results:Five founders of miR-106b transgenic mice were established and one high-level expression line was identified. Compared with the wild type mice, the expression of TGFBR2 was increased in miR-106b transgenic mice. Conclusion:miR-106b transgenic mouse has been established and it can be used to investigate the function of miR-106b on the development of Alzheimer’s disease.