An updated perspective on the polymerase division of labor during eukaryotic DNA replication |
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Authors: | Thomas A Guilliam |
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Institution: | 1. Division of Protein and Nucleic Acid Chemistry, Medical Research Council Laboratory of Molecular Biology, Cambridge, UK jyeeles@mrc-lmb.cam.ac.uk guilliam@mrc-lmb.cam.ac.ukhttps://orcid.org/0000-0003-0065-2693 |
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Abstract: | Abstract In eukaryotes three DNA polymerases (Pols), α, δ, and ε, are tasked with bulk DNA synthesis of nascent strands during genome duplication. Most evidence supports a model where Pol α initiates DNA synthesis before Pol ε and Pol δ replicate the leading and lagging strands, respectively. However, a number of recent reports, enabled by advances in biochemical and genetic techniques, have highlighted emerging roles for Pol δ in all stages of leading-strand synthesis; initiation, elongation, and termination, as well as fork restart. By focusing on these studies, this review provides an updated perspective on the division of labor between the replicative polymerases during DNA replication. |
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Keywords: | DNA replication replisome DNA polymerase polymerase delta replication fork |
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