The effect of Bacillus subtilis producing Surfactin in ROS production and transformation efficiency of tobacco cells |
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| Authors: | F Nazari BM Soltani M Shams-Bakhsh M Sharifi |
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| Institution: | 1. Department of Plant Pathology, Tarbiat Modares University, Tehran, Iran;2. Department of Molecular Genetics, Tarbiat Modares University, Tehran, Iran;3. Department of Plant Physiology, Tarbiat Modares University, Tehran, Iran |
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| Abstract: | Surfactin secreted by bacilli has biological functions in plant. Surfactin C14 and C15 have the highest effect on inducing hydrogen peroxide species release in the plant. Surfactin production in the two Bacillus strains ACCT21332 and FKR3 were analysed by HPLC and the phytotoxicity of the Bacilli-derived surfactins was determined in Tobacco cell culture. Surfactin C14 and C15 were detected in ACCT21332 but not in FKR3 strain. Extracellular hydrogen peroxide produced by tobacco cell culture cells exposed to ATCC21332 and FKR3 strains increased compared to untreated ones. The Agrobacterium mediated transformation rate of tobacco cells drops from 4% transformed cells to 0.8 and 1.2% when pretreated with ATCC21332 or FKR3 strain, respectively. The strong drop in transformation rate of plant cell culture after FKR3 strain pre-treatment indicates that Surfactin C14 and C15 are not the major or the only cause in protecting plant cells from Agrobacterial infection and transformation. |
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| Keywords: | Surfactin lipopeptide HPLC Bacillus subtilis transformation |
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