Cloning and sequencing of PR5-like gene from high altitude adapted ecotype of Lepidium latifolium

PR5-like gene (LlPR5) identified from substracted cDNA library of Lepidium latifolium in response to cold stress was characterised. Based on the EST sequence (FG618347) of a cDNA fragment, the full-length cDNA of 1422 bp was cloned by rapid amplification of cDNA ends. This LlPR5 has 931 bp of CDS encoding a protein of 326 amino acids with molecular weight of 34.46 kDa. Phylogenetic and conserved motif analysis reveals that cloned LlPR5 gene of L. latifolium formed cluster with At1g75800 of Arabidopsis thaliana. In silico promoter analysis of homolog of LlPR5 gene from Arabidopsis genome identified cis elements with possible role in regulation of cold/low temperature response in addition to its role in various stresses induced by pathogens in the plants.