Redox modulation of reductase and phosphatase activities in human erythrocytes |
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Authors: | F Marques M E Crespo Z I Silva M Bicho |
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Institution: | (1) Departamento de Radioisótopos, Instituto de Técnicas Nucleares, Lisbon;(2) Laboratório de Endocrinologia, Institute Portugus Oncologia de Francisco Gentil, Lisbon;(3) Laboratório de Genética, Faculdade de Medicina, Universidade de Lisboa, Av. Professor Egas Moniz, P-1699 Lisboa, Portugal |
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Abstract: | Summary We have previously reported that ferricyanide reductase activity in human erythrocytes depended on glycolysis and could be modulated by several compounds including oxidants and hormones like insulin. Insulin could activate glycolysis, probably as a consequence of tyrosine phosphorylation of protein band 3, implicating phosphorylation reactions as an important signal for activation of the reductase by insulin. Reversible phosphorylation of cellular proteins is also believed to play a key role in the action of insulin. Cytosolic acid phosphatase activity has been found in human erythrocytes. To further extend initial reports, we studied the effect of modulators on the cytosolic erythrocyte acid phosphatase. Mild oxidants like ferricyanide (1 mM), vanadate (1 mM), Mn2+ (0.5 and 1 mM), and phenylarsine oxide (10 and 100 M) inhibited the phosphatase activity. Similarly, insulin at concentrations that stimulate ferricyanide reduction (500, 1000 IU/ml) inhibited the activity of the phosphatase enzyme. The overall results indicated that oxidants are able to inhibit the acid phosphatase and stimulate the redox enzyme. In addition, a significant negative correlation (r = –0.400; P = 0.006) was observed between phosphatase and reductase activities. The observations discussed here, together with previous ones, emphasize that a close association between reductase and phosphatase enzymes may exist and also suggest a role for redox reactions in tyrosine phosphorylation/dephosphorylation-mediated signal transduction pathways. |
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Keywords: | Human erythrocytes Protein tyrosine kinases Protein tyrosine phosphatases Redox-active species Redox enzymes Signal transduction |
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