Androgenic response to preculture stress in microspore cultures of barley |
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Authors: | S Oleszczuk S Sowa J Zimny |
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Institution: | (1) Department of Plant Biotechnology and Cytogenetics, Institute of Plant Breeding and Acclimatization, Blonie |
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Abstract: | Summary. Various stresses such as starvation and cold or heat shocks have been identified as triggers in the induction of the microspore
embryogenesis. This study attempts to quantify the effects of different pretreatment conditions for successful microspore
culture of malting barley (cv. Scarlett). While the sporophytic microspore development could be induced from treated and nontreated
microspores, abiotic stress was essential for embryo formation and plant regeneration. The type of stress treatment applied
affected the numbers and the ratios of albino and green plants regenerated, as well as their fertility. The highest number
of green plants was obtained after the treatment of anthers in 0.3 M mannitol at 32 °C for 24 h before microspore culture.
Correspondence and reprints: Department of Plant Biotechnology and Cytogenetics, Institute of Plant Breeding and Acclimatization,
Radzików, 05-870 Blonie, Poland. |
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Keywords: | : Androgenesis Microspore culture Doubled haploid |
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