Microtubules regulate the organization of actin filaments at the cortical region in root hair cells ofHydrocharis |
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Authors: | M Tominaga K Morita S Sonobe E Yokota T Shimmen |
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Institution: | (1) Department of Life Science, Faculty of Science, Himeji Institute of Technology, Harima Science Park City, 678-12 Hyogo, Japan |
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Abstract: | Summary We studied the mechanism controlling the organization of actin filaments (AFs) inHydrocharis root hair cells, in which reverse fountain streaming occurs. The distribution of AFs and microtubules (MTs) in root hair
cells were analyzed by fluorescence microscopy and electron microscopy. AFs and MTs were found running in the longitudinal
direction of the cell at the cortical region. AFs were observed in the transvacuolar strand, but not MTs. Ultrastructural
studies revealed that AFs and MTs were colocalized and that MTs were closer to the plasma membrane than AFs. To examine if
MTs regulate the organization of AFs, we carried out a double inhibitor experiment using cytochalasin B (CB) and propyzamide,
which are inhibitors of AFs and MTs, respectively. CB reversibly inhibited cytoplasmic streaming while propyzamide alone had
no effect on it. However, after treatment with both CB and propyzamide, removal of CB alone did not lead to recovery of cytoplasmic
streaming. In these cells, AFs showed a meshwork structure. When propyzamide was also removed, cytoplasmic streaming and the
original organization of AFs were recovered. These results strongly suggest that MTs are responsible for the organization
of AFs inHydrocharis root hair cells. |
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Keywords: | Root hair cell Cytoplasmic streaming Actin filament Microtubule Cytochalasin B Propyzamide |
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