The endomembrane system and mechanism of membrane flow in the green alga,Gloeomonas kupfferi (Volvocales,Chlorophyta) II. A cytochemical analysis |
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Authors: | D S Domozych |
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Institution: | (1) Department of Biology, Skidmore College, Saratoga Springs, New York |
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Abstract: | Summary Cytochemical analysis of the endomembrane system of the chlamydomonad flagellate,Gloeomonas kupfferi (Chlorophyta), reveals distinct compartmentalization. Phosphatase localization shows that: IDPase is located throughout all cisternae of the dictyosome and vesicles associated with the contractile vacuole. Other alkaline phosphatases like TPPase, ATPase and ITPase were localized within the trans-face cisternae and vesicles of the contractile vacuole. IMPase was localized at the plasmamembrane and not within the endomembrane system. Acid phosphatases, incl. CMPase, NADPase and -glycerophosphatase, were localized in vesicles emerging from the central terminus of the trans-face of the dictyosome and in the peripheral vacuolar network. Silver proteinate labeling was noted in the dictyosome, contractile vacuole and on the anterior plasmamembrane. A summary of endomembrane compartmentalization and a putative interpretation of membrane flow and economy are presented.Abbreviations ER
endoplasmic reticulum
- IDPase
inosine 5-diphosphatase
- ITPase
inosine 5-triphosphatase
- ATPase
adenosine 5-triphosphatase
- TPPase
thiamine pyrophosphatase
- CMPase
cytidine 5-monophosphatase
- NADPase
-nicotinamide adenine diphosphatase
- AcPase
acid phosphatase |
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Keywords: | Endomembrane Cytochemistry Phosphatase Dictyosome Gloeomonas |
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