Turgor regulation and cytoplasmic free Ca2+ in the algaLamprothamnium |
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Authors: | Yoshiji Okazaki Yasuaki Yoshimoto Yukio Hiramoto Masashi Tazawa |
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Institution: | (1) Department of Biology, Faculty of Science, University of Tokyo, Hongo 7-3-1, 113 Tokyo, Japan;(2) Department of Cell Biology, National Institute for Basic Biology, Okazaki, 444 Aichi, Japan;(3) Biological Laboratory, Tokyo Institute of Technology, 152 Tokyo, Japan;(4) Present address: Department of Biology, Osaka Medical College, Sawaragi-cho 2-41, 569 Osaka, Japan;(5) Present address: The University of the Air, Wakaba 2-11, 260 Chiba, Japan |
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Abstract: | Summary In internodal cells ofLamprothamnium succinctum, turgor regulation in response to hypotonie treatment is inhibited by lowering external Ca2+ concentration (Ca2+]e) from 3.9 (normal) to 0.01 (low) mM. In order to clarify whether a change in the cytoplasmic free Ca2+ concentration (Ca2+]c) is involved in turgor regulation, the Ca2+ sensitive protein aequorin was injected into the cytoplasm of internodal cells. A large transient light emission was observed upon hypotonic treatment under normal Ca2+]e but not under low Ca2+]e. Thus hypotonic treatment induces a transient increase in Ca2+]c under normal Ca2+]e but not under low Ca2+]e.Abbreviations ASW
artificial sea water
- i
cellular osmotic pressure
- Ca2+]c
cytoplasmic free Ca2+ concentration
- EDTA
ethylenediamine-tetraacetic acid
- EGTA
ethylenglycol-bis(-aminoethyl ether(N,N-tetraacetic acid
- Ca2+]e
external Ca2+ concentration
- e
external osmotic pressure
- GM
glass micropipette
- GP
glass plate
- HEPES
N-2-hydroxyethylpiperazine-N-2-ethansulfonic acid
- MS
microscope stage
- OL
objective lens
- PIPES
piperazine-N-N-bis(2-ethanesulfonic acid)
- W
Weight |
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Keywords: | Aequorin Calcium Characeae Lamprothamnium Turgor regulation |
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