| 人免疫缺陷病毒I型抗AZT抗药株的体外研究 |
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| 引用本文： | 董关木.人免疫缺陷病毒I型抗AZT抗药株的体外研究[J].微生物学免疫学进展,1994(4). |
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| 作者姓名： | 董关木 |
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| 作者单位： | 中国药品生物制品检定所 |
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| 摘 要： | 人免疫缺陷病毒Ⅰ型（ＨＩＶ－１）抗３＇─叠氮─３＇─脱氧胸腺嘧啶（ＡＺＴ）抗药株经体外感染Ｃ８１６６淋巴细胞在高浓度ＡＺＴ条件下筛选获得，并暂命名为ＨＩＶ─１─Ｒ株。该抗药株与ＨＴＬＶ─ⅢＢ株相比，在同一感染复数（Ｍ０１）病毒量感染Ｃ８１６６细胞，经不同浓度的ＡＺＴ处理后，其复制的病毒量和对ＡＺＴ的敏感性有显著的差异，抗药株感染Ｃ８１６６细胞，加ＡＺＴ处理后，分离细胞ＤＮＡ作ＰＣＲ扩增后分析，特异性病毒的ＤＮＡ量比敏感株高１００倍以上，显示其抗药性作用点在病毒逆转录ＤＮＡ前。
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| 关 键 词： | 3＇─叠氮─3＇─脱氧胸腺嘧啶，多聚酶链反应，人免疫缺陷病毒抗药株 |
Study of HIV-1 Resistant to AZT Virus Strain in Vitro |
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| Dong Guanmu, Cuido Antonelli.Study of HIV-1 Resistant to AZT Virus Strain in Vitro[J].Progress In Microbiology and Immunology,1994(4). |
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| Authors: | Dong Guanmu Cuido Antonelli |
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| Abstract: | 3'-Azido-3'-deoxythmidine (AZT) resistant strain of human immunodeficiency virus type-1(HIV-1)was obtained by growing HTLV-Ⅲ B strain in C8166 cells culture in the presence of inhibitory concentration of AZT. Resistant'strain revealed that infectious virus yield and degrees of sensitivity to AZT were drastic different from HTLV-ⅢB during propagation in C8166 cells in the presence of the drug.PCR experiments performed on DNA extracted from C8166 cells infected with a resistant revealed that viral DNA was produced in presence of inhibitory concentration of AZT. It was revealed high resistance drug character. |
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| Keywords: | 3'-Azido-3'-deoxythmidine (AZT) PCR AZT-resistant HIV-1 |
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