分枝杆菌制剂对小鼠的免疫调节作用的初步研究

探讨不同分枝杆菌制剂对小鼠的免疫调节作用。将C57BL/6小鼠,随机分成4组分别注射生理盐水、微黄分枝杆菌、草分枝杆菌和田鼠分枝杆菌制剂,每隔2周免疫一次。免疫2次后,小鼠取脾淋巴细胞体外培养,分别以PPD、PPDB刺激,用MTT法检测T淋巴细胞增殖情况;用酶联免疫斑点法(Enzyme-Linked ImmunoSpot assay,ELISpot)检测脾细胞分泌IFN-γ情况,分析各分枝杆菌制剂对小鼠免疫应答的影响。结果显示,与对照组相比,微黄分枝杆菌制剂和草分枝杆菌制剂免疫小鼠的T淋巴细胞增殖反应和脾细胞分泌IFN-γ均明显提高,其中微黄分枝杆菌组与对照组相比有显著性差异(p<0.01)。分枝杆菌制剂可促进小鼠免疫应答。

The immune regulation of different Mycobacterium preparations in mice

To approach the regulation of different Mycobacterium preparations on the cell-mediated immunity of normal mouse.Divide C57BL/6 mice into different groups(N.S、M.phl、M.mic and M.fla) randomly,and immunize animals of different groups with different preparations once a week.After 2 injections,splenic lymphocytes were isolated sterilely,cultured in vitro and stimulated with PPD and PPDB;T lymphocyte proliferation was measured with MTT,and the IFN-γ secreted by T lymphocytes was measured with enzyme-linked immunospot assay;analyze the influence of different Mycobacterium preparations on the cell-mediated immunity of mouse.The fact that M.fla and M.phl preparations can enhance T lymphocyte proliferation and increase IFN-γ secretion of T lymphocyte indicates a promotion on mouse cell-mediated immunity.Mycobacterium preparations can up-regulate the cell-mediated immunity of mouse.