| 检测特异性抗体分泌细胞(ASCs)的固相酶联免疫斑(ELISPOT)技术的建立 |
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| 引用本文： | 谢贵林,田玉珍,杨吉明,王秉瑞.检测特异性抗体分泌细胞(ASCs)的固相酶联免疫斑(ELISPOT)技术的建立[J].微生物学免疫学进展,1994(1). |
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| 作者姓名： | 谢贵林 田玉珍 杨吉明 王秉瑞 |
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| 作者单位： | 卫生部兰州生物制品研究所 |
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| 摘 要： | ＥＬＩＳＰＯＴ技术是目前国外评价疫苗免疫原性和免疫应答机理研究中最常使用的方法。本文用自制的ＮＣ膜２４孔板为固相支持物，建立了检测小鼠脾细胞中特异性ＡＳＣｓ的ＥＬＩＳＰＯＴ技术，在检测方法的特异性、结果的稳定性上均取得了满意的效果。并对该方法的某些实验条件进行了摸索。
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| 关 键 词： | NC膜24孔板制备，抗体分泌细胞，固相酶联免疫斑点 |
Development of a Solid-Phase Enzyme-Linked Immunospot Assay for Enumeration of Specific Antibody-Secreting Cells |
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| Xie Guilin et al.Development of a Solid-Phase Enzyme-Linked Immunospot Assay for Enumeration of Specific Antibody-Secreting Cells[J].Progress In Microbiology and Immunology,1994(1). |
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| Authors: | Xie Guilin |
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| Abstract: | A solid-phase enzyme-linked immunospot assay (ELISPOT) is described for enumeration of mouse splenocytes secreting specific antibody with nitrocellulose bottomed 24-well plates made by our laboratory. This nitrocellulose-bottomed solid-phase readily absorb a variety of antigens and has excellent specificity. The optimal conditions for detection of mouse splenocytes secreting IgG antibody are described. |
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| Keywords: | ELISPOT ASCs nitrocellulose-bottomed 24-well plates |
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