| 重组CHO细胞乙肝表面抗原诱导小鼠细胞免疫应答的研究 |
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| 引用本文： | 叶琳,张鹏艳,黄林.重组CHO细胞乙肝表面抗原诱导小鼠细胞免疫应答的研究[J].微生物学免疫学进展,2007,35(4):6-9. |
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| 作者姓名： | 叶琳 张鹏艳 黄林 |
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| 作者单位： | 成都生物制品研究所,成都,610023 |
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| 摘 要： | 为了研究重组CHO细胞乙肝表面抗原(CHO-rHBsAg)在小鼠中诱导T细胞免疫应答的能力,全面评价疫苗的免疫原性,以CHO-rHBsAg免疫BALB/c小鼠,常规制备小鼠脾脏淋巴细胞并在体外以抗原或特异多肽刺激;采用ELISA法测定抗原特异性T淋巴细胞分泌的细胞因子,乳酸脱氢酶法(LDH)测定抗原特异性细胞毒T淋巴细胞(CTL)活性,酶联斑点法(ELISPOT)测定CTL频数(CTLp),应用流式细胞仪分析T淋巴细胞亚群。结果显示,rHBsAg可在小鼠中诱导Th1及Th2类细胞因子;加铝佐剂的rHBsAg较未加佐剂的抗原可诱导较高水平的IFN-γ、CTL克隆及较高百分比的CD8+T淋巴细胞亚群。重组CHO细胞来源的HBsAg可在BALB/c小鼠中诱导一定程度的细胞免疫应答。
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| 关 键 词： | 重组乙肝表面抗原 细胞免疫 细胞毒T淋巴细胞 |
| 文章编号： | 1005-5673(2007)04-0006-04 |
| 修稿时间： | 2007年3月16日 |
Induction of cell-mediated immune response in BALB/c mice immunized with CHO cell derived recombinant HB-sAg |
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| YE Lin,ZHANG Peng-yan,HUANG Lin.Induction of cell-mediated immune response in BALB/c mice immunized with CHO cell derived recombinant HB-sAg[J].Progress In Microbiology and Immunology,2007,35(4):6-9. |
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| Authors: | YE Lin ZHANG Peng-yan HUANG Lin |
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| Abstract: | To study the response of T cell-mediated immunity(CMI) primed with CHO cell derived recombinant hepatitis B surface antigen(rHBsAg) in mice and evaluate the immunogenicity of vaccine,BALB/c mice were immunized by rHBsAg with or without adjuvant.Then spleen-lymphocytes were isolated and stimulated respectively by Ag or specific peptide for investigating CMI response.Cytokine assay,specific CTL activity,CTLp assay and the percentage of phenotypes in T cell were detected respectively by ELISA,LDH,ELISPOT and FCM.rHBsAg can trigger Th1 and Th2 type immune response.Comparing with naked rHBsAg,rHBsAg with Al(OH)3 adjuvant induced higher IFN-γ,more CTL clones and higher percentage of CD8+T cell population.CHO cell derived rHBsAg can induce CMI response to a certain extent in BALB/c mice. |
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| Keywords: | rHBsAg CMI CTL |
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