| 森林脑炎病毒疫苗株的全基因组序列分析 |
| |
| 引用本文： | 宋宗明,赵大鹏,刘双军,王晓宏,赵建,李彤.森林脑炎病毒疫苗株的全基因组序列分析[J].微生物学免疫学进展,2006,34(4):7-9. |
| |
| 作者姓名： | 宋宗明 赵大鹏 刘双军 王晓宏 赵建 李彤 |
| |
| 作者单位： | 长春生物制品研究所,长春,130062 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| |
| 摘 要： | 实验设计针对森林脑炎病毒的特异性引物,以被森林脑炎疫苗株病毒感染的鼠脑组织中提取的总RNA为模板,用PCR方法分段逆转录合成、扩增序列并测序,应用DNASTAR软件比较分析。结果表明,该病毒疫苗株的全基因组由10782个核苷酸组成,编码3414个氨基酸。森林脑炎疫苗株病毒全基因序列的测定,为研究该病毒疫苗株的生物学特性提供了分子基础。
|
| 关 键 词： | 森林脑炎病毒疫苗株 基因组 序列测定 |
| 文章编号： | 1005-5763（2006）04-0007-03 |
| 收稿时间： | 2006-06-13 |
| 修稿时间： | 2006-07-28 |
Sequencing of whole genome of Tick-borne encephalitis vaccine strain |
| |
| SONG Zong-ming, ZHAO Da-peng, LIU Shuang-jun ,et al..Sequencing of whole genome of Tick-borne encephalitis vaccine strain[J].Progress In Microbiology and Immunology,2006,34(4):7-9. |
| |
| Authors: | SONG Zong-ming ZHAO Da-peng LIU Shuang-jun |
| |
| Institution: | Changchun Institute of Biological Prodcts , Changchun 130062,China |
| |
| Abstract: | Sequencing of whole genome of Tick-borne encephalitis vaccine(TBEV) strain was carried out in this study,the total RNA was extracted from the mice infected with TBEV strain,and amplified by PCR based on designed primers.The RT-PCR products of the RNA were purified,sequenced and analyzed by DNASTAR software.The result shows that the whole genome is consisted of 10782 nucleotides encoding 3414 amino acids respectively.It laid a molecular foundation for the investigation of biological characters of TBEV strain. |
| |
| Keywords: | Tick-borne encephalitis vaccine(TBEV) strain Genome Sequencing |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
