大流行流感疫苗血清学检测中马血球和鸡血球血凝抑制试验方法的比较 |
| |
引用本文: | 李长贵,邵铭,于丹,刘书珍,高强,方捍华.大流行流感疫苗血清学检测中马血球和鸡血球血凝抑制试验方法的比较[J].微生物学免疫学进展,2008,36(4). |
| |
作者姓名: | 李长贵 邵铭 于丹 刘书珍 高强 方捍华 |
| |
作者单位: | 1. 中国药品生物制品检定所,北京,100050 2. 北京科兴生物制品有限公司,北京,100085 |
| |
摘 要: | 血凝抑制试验(HI)是评价季节性流感疫苗免疫效果的经典方法。由于对人、禽流感病毒的受体不同,不同来源的红血球检测敏感性可能有差异。实验中比较了经典的鸡血球HI方法和国外报道的马血球HI方法,发现两种方法在检测大流行流感疫苗接种者血清时,于不同毒株抗原检测时表现各不相同,检测结果差异在2倍之内,说明经典的鸡血球HI方法仍适用于评价大流行流感疫苗的免疫效果。
|
关 键 词: | 大流行流感疫苗 血凝抑制试验 马血球 鸡血球 |
Comparison of horse and chicken red cells-based hemagglutination inhibition(HI) assays for serological evaluation of pandemic influenza vaccines |
| |
Abstract: | The hemagglutination-inhibition(HI) assay is a traditional method for assessing immune responses to influenza virus.It is well known that the receptors for avian and human influenza virus on the host cells are different;therefore,HI test based on red cells from various species of animals containing different receptors may have different sensitivity.In this study,The traditional chicken red cells-based HI(cHI) with horse red cells based-HI(hHI) methods was compared in the detection of antibodies against H5N1 influenza virus.It found that two kinds of method have different performances when 3 kinds of HI antigens were used,but the differences between two assays were roughly within the 2-fold range.Therefore,the traditional chicken red cells-based HI is still suitable for the serological evaluation of pandemic influenza vaccines. |
| |
Keywords: | Pandemic influenza vaccines Hemagglutination-inhibition(HI) assay Horse red cells Chicken red cells |
本文献已被 维普 万方数据 等数据库收录! |
|