狂犬病毒aG株在Vero细胞上传代适应研究

为研制有效、安全和稳定的Vero细胞狂犬病疫苗提供实验室基础资料。采用狂犬病固定毒4aG株在Vero细胞上进行传代适应,同时对该毒株在Vero细胞上的增殖条件,病毒液的回收方法进行研究。结果显示,狂犬病固定毒4aG株在Vero细胞上多次传代后获得一株Vero细胞适应株(4aG-V株),该毒株的毒力可达8.50 logLD50/ml,且具有很好的抗原性及免疫原性。结果表明,感染Vero细胞最适种毒比例为1∶103,病毒滴度随着时间的延长而增强到第12天后逐渐减弱,且采用低温冻融破碎法回收的病毒液滴度优于直接收液法。4aG-V株在Vero细胞上维持时间长,可连续收液,有望其生产高滴度的狂犬病毒液。

Adaptation of rabies virus aG strain in Vero cells

To provide experimental basis for the preparation of effective,safe and stable rabies vaccine with Vero cells.Subculture fixed rabies virus strain 4aG in Vero cells and explore the condition for virus propagation as well as the method for harvest of virus liquid.After subculture of 4aG strain in Vero cells,an adapted rabies virus strain 4aG-V was obtained,which reached a virulence of 8.50 log LD50/ml and showed that it is of the good antigenicity and immunogenicity.The optimal MOI of 4aG strain in Vero cells is 1∶103.The virus titer increase with the culture time within 12 d and then decrease gradually.Comparing with the titer of virus harvested directly,that of virus liquid harvested by freeze-thaw at low temperature is significantly high.The virus liquid of 4aG-V strain cultured in Vero cells may be harvested for several times,indicating that the strain is suitable for the production of high titer bulk rabies virus.