| 聚丙烯酰胺凝胶电泳中LPS样品制备方法的改进 |
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| 引用本文： | 杨朝晖,计永莉,周园,王军,赵志强.聚丙烯酰胺凝胶电泳中LPS样品制备方法的改进[J].微生物学免疫学进展,2007,35(4):38-41. |
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| 作者姓名： | 杨朝晖 计永莉 周园 王军 赵志强 |
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| 作者单位： | 兰州生物制品研究所,兰州,730046 |
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| 摘 要： | 为了对革兰阴性细菌的LPS进行快速分析,观察LPS的产量、分子量分布,进行菌种筛选和抗原传代稳定性研究,实验中对热酚法、酶水解法、冷酚法三种制备LPS的方法进行了比较。结果证明,改进的水饱和冷酚LPS提取方法,需要时间短、经济、方便,只须几个细菌菌落就可以提取LPS进行SDS-PAGE电泳分析,说明该方法有很好的实用性。
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| 关 键 词： | 脂多糖 SDS-PAGE 抽提 |
| 文章编号： | 1005-5673(2007)04-0038-04 |
| 修稿时间： | 2007年7月30日 |
The study on the modified cold water-saturation phenol LPS extract method for SDS-PAGE compared with other two methods |
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| YANG Zhao-Hui,JI Yongli,ZHOU Yuan,et al..The study on the modified cold water-saturation phenol LPS extract method for SDS-PAGE compared with other two methods[J].Progress In Microbiology and Immunology,2007,35(4):38-41. |
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| Authors: | YANG Zhao-Hui JI Yongli ZHOU Yuan |
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| Abstract: | To rapidly analysis and observe the molecular character of Gram negative bacteria LPS,select bacteria strain for polysaccharide preparation,study the stability of O-antigen during the bacteria strain passage,Three kinds LPS extract methods hot-phenol extract,enzyme-hydrolysis,and cold water-saturation phenol extract are compared.The results showed that the modified cold water-saturation phenol extract method is the best one,It is of the advantage of time saving,economics,convenient and higher yields.This modified method can be applied widely. |
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| Keywords: | SDS-PAGE |
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