| 重组人睫状神经营养因子蛋白质定量检测方法的建立 |
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| 引用本文： | 马亚茹,陆俭,李萍,高雪峰,蒋琳.重组人睫状神经营养因子蛋白质定量检测方法的建立[J].微生物学免疫学进展,2011,39(3):29-32. |
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| 作者姓名： | 马亚茹 陆俭 李萍 高雪峰 蒋琳 |
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| 作者单位： | 兰州生物制品研究所,兰州,730046 |
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| 摘 要： | 采用ELISA双抗体夹心法,建立一种快速灵敏的定量检测rhCNTF成品蛋白含量的方法。结果显示,rhC-NTF抗原浓度在(0～25)ng范围内线性良好(r>0.99),灵敏度为0.3ng/ml,与其他重组细胞因子无交叉反应,样品的检测结果与理论含量相吻合,CV<15%,该方法检测速度快、重复性好、灵敏度高、特异性好。
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| 关 键 词： | 重组人睫状神经营养因子 双抗体夹心ELISA法 |
The establishment of the method for quantitative detection of rhCNTF protein |
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| MA Ya-ru,LU Jian,LI Ping,et al..The establishment of the method for quantitative detection of rhCNTF protein[J].Progress In Microbiology and Immunology,2011,39(3):29-32. |
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| Authors: | MA Ya-ru LU Jian LI Ping |
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| Institution: | MA Ya-ru,LU Jian,LI Ping,et al.(Lanzhou Institute of Biological Products,Lanzhou 730046,China) |
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| Abstract: | A rapid and sensitive method for quantitative detection of rhCNTF protein was established by double antibody sandwich ELISA.The results showed that the linearity detection range of the rhCNTF concentration is 0-25 ng/ml(r>0.99),the sensitivity of the rhCNTF ELISA with a limit of detection is 0.3 ng/ml,it is of no cross reaction with other recombinant cytokines.The tested results of samples are tallied with the theoretical content data,CV(coefficient of variation) <15%.This method is of the advantage of rapi... |
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| Keywords: | rhCNTF Double antibody sandwich ELISA |
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