热休克蛋白HSP65嵌合表达系统的构建和表达 |
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引用本文: | 雷清,蒋琳,马超,刘鹏.热休克蛋白HSP65嵌合表达系统的构建和表达[J].微生物学免疫学进展,2007,35(3):24-26. |
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作者姓名: | 雷清 蒋琳 马超 刘鹏 |
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作者单位: | 兰州生物制品研究所,兰州,730046 |
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摘 要: | 设计一条含(GlySer)3连接肽的引物,从BCG经PCR得到HSP65-Linker的基因片段。将该基因定向克隆入原核表达载体pET42b(+),用大肠杆菌BL21(DE3)转化,构建了pET42-LHSP65的嵌合表达载体。DNA测序正确后经IPTG诱导表达,得到可溶性目的蛋白,Western-blot也证实了此重组蛋白可与抗HSP65发生特异性免疫反应。此重组体的构建为进一步探索HSP65的免疫佐剂功效奠定了基础。
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关 键 词: | HSP65 连接肽 基因表达 |
文章编号: | 1005-5673(2007)03-0024-03 |
修稿时间: | 2006年12月28 |
Expression and construction of chimeric vector about HSP65 |
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Abstract: | A primer including(Gly4Ser)3was designed,The LHSP65 gene was amplified by PCR from BCG,then ligated into expression vector pET42b(+) directly,Which was transformed into E.coli BL21(DE3) and obtained pET42-LHSP65 expression vector.It was detected by sequencing DNA and induced with IPTG.The recombinant protein is soluble,Western blotting analysis showed that the protein can react with anti-HSP65 antibody specifically.This chimeric vector is established for researching HSP65 protein. |
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Keywords: | HSP65 |
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