| 绿色荧光报告基因在细胞免疫检测中的应用 |
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| 引用本文： | 尤崇革,周旭,侯玲,李益民.绿色荧光报告基因在细胞免疫检测中的应用[J].微生物学免疫学进展,2002,30(1):42-43. |
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| 作者姓名： | 尤崇革 周旭 侯玲 李益民 |
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| 作者单位： | 1. 兰州医学院第二附属医院
2. 兰州生物制品所,兰州,730030 |
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| 摘 要： | 将丙肝病毒C E1区基因插入绿色荧光报告基因pEGFP-N1中，构建真核表达重组质粒pEGFP-N1-HCV/C E1。转染小鼠骨髓瘤细胞SP2/0，在荧光显微镜下观察绿色荧光融合蛋白的表达情况。结果在细胞浆中出现了绿色荧光，表明目的基因得到表达，再通过G418筛选后大量培养用作细胞毒实验的靶细胞，结果表明以EGFP报告基因作筛选标记制备的靶细胞完全可以满足细胞毒实验要求。
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| 关 键 词： | 绿色荧光蛋白报告基因 丙肝病毒 细胞毒实验 细胞免疫检测 应用 |
| 文章编号： | 1005-5673(2002)-01-0042-02 |
| 修稿时间： | 2001年6月9日 |
Application of green fluorescent protein fusion vector in detecting cellular immune response |
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| YOU Chong ge,ZHOU Xu,HOU Ling,et al..Application of green fluorescent protein fusion vector in detecting cellular immune response[J].Progress In Microbiology and Immunology,2002,30(1):42-43. |
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| Authors: | YOU Chong ge ZHOU Xu HOU Ling |
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| Abstract: | A HCV/C+E 1 DNA fragment encoding core and envelope(E 1)proteins was inserted into a green fluorescent protein fusion vector to construct an eukaryotic expression recombinant plasmid,which has been transfected into mouse myeloma cell line SP2/0 by FuGENE TM 6 transfection reagent in vitro,and the green fluorescent emited by UV in SP2/0 cells was observed.It indicated the successful expression of aim genes.After screening with G 418 ,the transfected SP2/0 cells were cultured in a large amount as the target cell in detection of CTL activity.The result of cytotoxicity test suggested that EGFP could be made as a screening marker to apply to the preparation of target cell in detection of CTL activity. |
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| Keywords: | Green fluorescent protein fusion vector Hepatitis c virus CTL activity |
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